Summary
The role of Ca2+ in protein secretion from the isolated perfused cat's pancreas, the effect of the dibutyryl analogues of cAMP and cGMP, and the interrelation of Ca2+ and the nucleotides were studied. The following results were obtained:
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1.
Pancreatic enzyme secretion can be elicited by CaCl2 injections into the pancreatic arteries and is linearily related to the peak Ca2+ concentration in the effluent perfusate. Different background Ca2+ concentrations in the perfusate (3 mM or 0.125 mM) do not disturb this relation, indicating that no adaptation occurs. The effect of Ca2+ injections is of the same magnitude as that evoked by the hormones pancreozymin or acetylcholine.
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2.
Injections of Ca2+ potentiate the effects of dibutyryl adenosine 3′,5′-cyclic monophosphate (dbcAMP), dibutyryl guanosine 3′,5′-cyclic monophosphate (dbcGMP) or theophylline.
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3.
Infusion of low doses of pancreozymin increases the Ca2+ effect.
The findings indicate that extracellular Ca2+ is involved in the mechanism of enzyme secretion and that Ca2+ and cyclic nucleotides have a synergistic action on the target.
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Schulz, I., Mannigel, H., Christian, A.L. et al. The role of extracellular Ca2+ and cyclic nucleotides in the mechanism of enzyme secretion from the cat pancereas. Pflugers Arch. 360, 165–181 (1975). https://doi.org/10.1007/BF00580539
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DOI: https://doi.org/10.1007/BF00580539